Gene description for Flot2
Gene name flotillin 2
Gene symbol Flot2
Other names/aliases Esa
reggie-2
Species Mus musculus
 Experiment description of studies that identified Flot2 in Primary cortical neurons

1
Experiment ID1629
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SamplePrimary cortical neurons
Sample nameC57BL/6
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyWestern blotting
LC-MS
EV-TRACK -

2
Experiment ID1630
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SamplePrimary cortical neurons
Sample nameC57BL/6
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyWestern blotting
LC-MS
EV-TRACK -

3
Experiment ID1638
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SamplePrimary cortical neurons
Sample nameC57BL/6
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID1639
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 29348617   
OrganismMus musculus
Experiment descriptionNeuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures
AuthorsMiranda AM, Lasiecka ZM, Xu Y, Neufeld J, Shahriar S, Simoes S, Chan RB, Oliveira TG, Small SA, Di Paolo G.
Journal name Nat Commun
Publication year2018
SamplePrimary cortical neurons
Sample nameC57BL/6
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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